Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 2
Release date: Aug 17 2007
Last update date: Aug 28 2018
Access: Public
Dataset link Transcription profiling by array of Arabidopsis after hypoxia treatment

Experimental Protocol


For the hypoxic treatment protoplasts were submerged in mannitol buffer and the hypoxia condition was confirmed by the activation of the well-established early marker genes ADH (alcohol dehydrogenase, At1g77120) and PDC1 (pyruvate decarboxylase-1, At4g33070) based on RT-PCR and GeneChip® analyses. Results show a clear overlap in genes induced under hypoxic conditions and those induced by KIN10 expression in protoplasts. The overlap of the gene expression profiles was confirmed by triplicated real-time quantitative RT-PCR analyses of selected marker genes.

Repositories


GEO

GSE8248

ArrayExpress

E-GEOD-8248

BioProject

PRJNA101191

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Contact


Elena Baena-Gonzalez