Computational protocol: Mutation of Serine 1333 in the ATR HEAT Repeats Creates a Hyperactive Kinase

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Protocol publication

[…] Site directed mutagenesis of ATR in a modified pCDNA5/TO FLAG-HA3 or pCDNA5/TO FLAG backbone was performed as previously described . Sequence alignments utilized ClustalW2 . The protein structure prediction was done with Phyre2 using ATR amino acids 1328–1364 for HEAT repeat 27 . […]

Pipeline specifications

Software tools Clustal W, Phyre
Application Protein structure analysis
Diseases Congenital Abnormalities