Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 16
Release date: Sep 17 2018
Last update date: Nov 29 2018
Access: Public
Dataset link Dynamic remodeling of translation programs drives hepatocyte proliferation during liver regeneration

Experimental Protocol


Hepatocytes were isolated in presence of Cycloheximide (CHX) from normal fed mice and DDC fed (4 week) mice. Polysomal fractions were separated over sucrose gradient and RNA was isolated from the three fractions Monosome (1 ribosome), Light polysome (2-4 ribosomes) and Heavy polysomes (>5). Isolated RNA was subject to polyA selection and sequenced on an Illumina platform.

Repositories


GEO

GSE106140

ENA

SRP121411

BioProject

PRJNA415722

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Contact


Sushant Bangru