Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 9
Release date: May 12 2010
Last update date: Oct 29 2018
Access: Public
Dataset link CD62L expression identifies a unique subset of polyfunctional CD56dim NK cells: Groups 1-3

Experimental Protocol


Gene expression profiles of FACSAria sorted CD3- CD56bright CD62L+, CD3- CD56dim CD62L+ and CD3- CD56dim CD62L- NK cells from human peripheral blood of three donors were compared using Affymetrix GeneChip Human Genome HG-U133_Plus_2. After total RNA extraction, reverse transcription, cDNA extraction, the biotinylated cRNA was transcribed, fragmented, and 15 µg cRNA hybridized in triplicates for each of the three groups to the GeneChip arrays. Group1: CD3- CD56bright CD62L+,.Group2: CD3- CD56dim CD62L+, Group3: CD3- CD56dim CD62L-. Lists of differentially regulated genes were created using High Performance Chip Data Analysis (HPCDA) with Bioretis database (http://www.bioretis-analysis.de). Worldwide data sharing is possible via Bioretis, please ask the authors.

Repositories


GEO

GSE21774

ArrayExpress

E-GEOD-21774

BioProject

PRJNA127297

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Contact


Pawel Durek
Joachim R. Grün

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