|Application:||Gene expression microarray analysis|
|Number of samples:||8|
|Release date:||Jul 1 2016|
|Last update date:||Aug 9 2018|
|Dataset link||Expression data from human bronchial epithelial cells|
Bronchial epithelial cells from cystic fibrosis patients homozygotes for F508del mutation were isolated. Cells differentiated as epithelial monolayers on porous membranes (Snapwell inserts) were incubated for 24 hours at 27 °C or kept under control conditions. Rescue of mutant CFTR channel by low temperature was checked by measuring transepithelial chloride currents with the Ussing chamber technique. Total RNA was extracted from treated and control cells to assess changes in gene expression with microarrays.