Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 8
Release date: Jul 1 2016
Last update date: Aug 9 2018
Access: Public
Diseases: Cystic Fibrosis
Mutations: p|DEL|508|F
Dataset link Expression data from human bronchial epithelial cells

Experimental Protocol


Bronchial epithelial cells from cystic fibrosis patients homozygotes for F508del mutation were isolated. Cells differentiated as epithelial monolayers on porous membranes (Snapwell inserts) were incubated for 24 hours at 27 °C or kept under control conditions. Rescue of mutant CFTR channel by low temperature was checked by measuring transepithelial chloride currents with the Ussing chamber technique. Total RNA was extracted from treated and control cells to assess changes in gene expression with microarrays.

Repositories


GEO

GSE70442

ArrayExpress

E-GEOD-70442

BioProject

PRJNA288692

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Contact


Diego Carrella