Dataset features

Specifications


Application: ChIP-seq analysis
Number of samples: 9
Release date: Sep 12 2014
Last update date: Sep 12 2014
Access: Public
Chemicals: Azoles
Dataset link Genomic targets sites of the Candida glabrata transcription factor Pdr1

Experimental Protocol


Examination of Pdr1 binding sites using two different antibodies in five different strains. Strains labeled TAP carry the TAP-tagged allele and capture was done with anti-TAP. The control was performed by carrying out a ChIP reaction on rho0 TAP-PDR1 cells but with no primary anti-TAP antibody. Since Pdr1 expression is the highest in this background, this was selected as the best control for antibody specificity. We also performed ChIP reactions on rho+, rho0 and cells lacking the PDR1 gene (pdr1D) cells using the anti-Pdr1 antibody. The control for these reactions was a ChIP experiment using anti-Pdr1 antibody on cells lacking the endogenous Pdr1 protein.

Repositories


GEO

GSE59839

ArrayExpress

E-GEOD-59839

ENA

SRP044948

BioProject

PRJNA256336

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Contact


Thomas Bair
Thomas B Bair