Computational protocol: Molecular Cloning and Functional Studies of Two Kazal-Type Serine Protease Inhibitors Specifically Expressed by Nasonia vitripennis Venom Apparatus

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Protocol publication

[…] The cDNAs and deduced amino acid sequences were analyzed by DNAStar software (version 5.02, DNAStar, Madison, Wisconsin, USA) and online Blast []. The signal peptides were analyzed by Signal P []. Multiple sequence alignments were carried out with DNAman software (Lynnon Biosoft, Quebec, QC, Canada) and Clustal W2 []. The phylogenetic tree was constructed by using MEGA 5.1 (Tokyo Metropolitan University, Tokyo, Japan) with the neighbor-joining (NJ) method. […]

Pipeline specifications

Software tools DNAMAN, Clustal W, MEGA
Application Phylogenetics
Organisms Nasonia vitripennis, Musca domestica