Dataset features

Specifications


Application: BS-seq analysis
Number of samples: 8
Release date: Jun 30 2013
Last update date: Oct 10 2018
Access: Public
Diseases: Hepatitis A
Chemicals: 5-Methylcytosine
Dataset link Vitamin C induces Tet-dependent DNA demethylation in ES cells to promote a blastocyst-like methylome [MeDIP-Seq]

Experimental Protocol


Oct4-GiP mouse embryonic stem (ES) cells were cultured in the presence or absence of Vitamin C (L-ascorbic acid 2-phosphate, 100 μg/ml) for 12 or 72 hours. Cells were maintained in N2B27 medium supplemented with LIF (1000 U/ml), MEK inhibitor PD0325901 (1 μM), and GSK3β inhibitor CHIR99021 (3 μM). Genomic DNA was used for DNA immunoprecipitation with antibodies against 5-hydroxymethylcytosine (hmC) or 5-methylcytosine (mC). Immunoprecipitated DNA was adaptor-ligated for paired-end sequencing on an Illumina HiSeq and sequence reads were aligned to the mm9 mouse reference genome for analysis.

Repositories


GEO

GSE46402

ArrayExpress

E-GEOD-46402

ENA

SRP021527

BioProject

PRJNA200282

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Contact


Martin Hirst

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