Computational protocol: MS_HistoneDB, a manually curated resource for proteomic analysis of human and mouse histones

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Protocol publication

[…] Multiple sequence alignments of mouse and human histones were performed using Clustal Omega []. Tree data were downloaded in aln format and displayed with iTOLv3 []. [...] Tissue-specific expression data were obtained from Huntley et al. [] through the Expression Atlas Repository []. RNA-seq data at different stages of spermatogenesis were obtained from da Cruz et al. []. Data were imported and treated in R using the pheatmap library (https://CRAN.R-project.org/package=pheatmap). […]

Pipeline specifications

Software tools Clustal Omega, PHeatmaps
Databases Expression Atlas
Applications Phylogenetics, RNA-seq analysis, Transcriptome data visualization
Organisms Homo sapiens, Mus musculus