Dataset features

Specifications


Application: ChIP-seq analysis
Number of samples: 2
Release date: Jul 1 2016
Last update date: Nov 17 2017
Access: Public
Diseases: Retinal Neoplasms, Intervertebral Disc Degeneration
Computational protocol: ea-utils, Bowtie2, MACS, BEDTools, i-cisTarget, MET, FlyBase
Dataset link Dachshund ChIP-seq in Drosophila Melanogaster 3rd instar imaginal eye discs

Experimental Protocol


ChIP-seq against Dachshund vs input ChIP-seq. Eye-antennal imaginal discs are dissected from Grh-GFP (Bloomington stock 42269) 3rd instar larvae and fixed with formaldehyde. Chromatin is prepared and sonicated until fragments reach an average size of 500 bp. Chromatin is immunoprecipitated with an anti-GFP Ab (ab290, Abcam) and the immunocomplexes are recovered with protein A/G magnetic beads (Millipore).

Repositories


GEO

GSE82151

ArrayExpress

E-GEOD-82151

ENA

SRP076015

BioProject

PRJNA324188

Download


Contact


Jelle Jacobs

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