Dataset features

Specifications


Dataset type: Methylation profiling by high throughput sequencing
Number of samples: 3
Release date: Jan 1 2019
Last update date: Feb 14 2019
Access: Public
Dataset link Trancriptomic and epigenetic analyses of mouse organoids expressing BrafV600E mutation [MBD-seq]

Experimental Protocol


Cre-activable BrafV600E and wild type proximal colon organoids were derived from mice carrying heterozygous Cre-activable BrafV600E (termed Braf+/LSL). Lentiviral delivery of Cre with the lentiviral backbone alone serving as control (EV), is used to subsequently activate BrafV600E in the organoids. Expression and methylation analyses were done in organoids 5m after induction of oncogene, and in organoids selected to grow in medium without stem-cell niche factors (Base medium). This Series represents the methylation analysis.

Repositories


GEO

GSE114795

ENA

SRP148768

BioProject

PRJNA472637

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