Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 62
Release date: Feb 21 2011
Last update date: Dec 6 2012
Access: Public
Chemicals: Abscisic Acid, Estrogens, Glucosinolates
Genes: FUS3
Dataset link Gene regulation by the Arabidopsis seed maturation master regulator FUS3

Experimental Protocol


This Series contains two different types of microarray experiments. First, seven-day-old seedlings of the ESTR-inducible FUS3 transgenic plants (ER-FUS3) were cultured for 3, 6, 12, 24, 36 or 48 h in a standard liquid medium or in that containing ESTR, ABA or both. RNA was prepared from each time point sample of each treatment and used to prepare Cy5-labeled cRNA, which was subsequently probed by DNA array with the reference Cy3-labeled cRNA prepared from the time zero untreated seedlings. Two biological replicates per treatment. Second, transcriptomes were compared between developing seeds of the wild-type (Col-0) and the fus3-3 mutant. This experiment contains two seed developmental stages, 8 days after flowering (DAF; bent cotyledon stage) and at 12 DAF (green cotyledon stage). Sample pairs in three biological replicates were analyzed by the two-color method. Dye-swapped hybridizations were performed in every replicate.

Repositories


GEO

GSE23974

ArrayExpress

E-GEOD-23974

BioProject

PRJNA130459

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Contact


Akiko Yamamoto