Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Oct 22 2014
Last update date: Aug 23 2018
Access: Public
Genes: WNT3, WNT3A
Dataset link Microarray of 3D cultured basal cells in the presence of Wnt3A

Experimental Protocol


Mammary glands from 8- to 12-week-old virgin female mice were isolated and single-cell suspension was obtained. Mammary stem cell enriched population (Lin-, CD24+, CD29hi) cells were isolated using BD FACSAria. The purity of sorted population was routinely checked and ensured to be more than 95%.Total RNA from 2nd colonies passage cultured in the presence of vehicle and Wnt3A was extracted with PicoPure (Arcturs) in accordance with the manufacturer’s protocol. RNA concentration was determined with NanoDrop ND-1000, and quality was determined using the RNA 6000 Nano assay on the Agilent 2100 Bioanalyzer (Agilent Technologies). Affymetrix microarray analysis, fragmentation of RNA, labelling, hybridization to Mouse Genome 430 2.0 microarrays, and scanning were performed in accordance with the manufacturer’s protocol (Affymetrix). Total 4 samples representing two sets of replicates were analyzed.

Repositories


GEO

GSE55117

ArrayExpress

E-GEOD-55117

BioProject

PRJNA238576

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Contact


Qing Cissy Yu