|Application:||Gene expression microarray analysis|
|Number of samples:||4|
|Release date:||Oct 22 2014|
|Last update date:||Aug 23 2018|
|Dataset link||Microarray of 3D cultured basal cells in the presence of Wnt3A|
Mammary glands from 8- to 12-week-old virgin female mice were isolated and single-cell suspension was obtained. Mammary stem cell enriched population (Lin-, CD24+, CD29hi) cells were isolated using BD FACSAria. The purity of sorted population was routinely checked and ensured to be more than 95%.Total RNA from 2nd colonies passage cultured in the presence of vehicle and Wnt3A was extracted with PicoPure (Arcturs) in accordance with the manufacturer’s protocol. RNA concentration was determined with NanoDrop ND-1000, and quality was determined using the RNA 6000 Nano assay on the Agilent 2100 Bioanalyzer (Agilent Technologies). Affymetrix microarray analysis, fragmentation of RNA, labelling, hybridization to Mouse Genome 430 2.0 microarrays, and scanning were performed in accordance with the manufacturer’s protocol (Affymetrix). Total 4 samples representing two sets of replicates were analyzed.
Qing Cissy Yu