Computational protocol: Changes in Retinal Function and Cellular Remodeling Following Experimental Retinal Detachment in a Rabbit Model

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Protocol publication

[…] All procedures were carried out in the right eyes while the left eyes served as controls. Animals were anesthetized by an intramuscular (IM) injection of ketamine hydrochloride (40 mg/kg) and xylazine (10 mg/kg) (Vetmarket, Shoham, Israel). The pupils were dilated with topical 0.5% phenylephrine hydrochloride, 0.5% tropicamide (Fischer Pharmaceutical Laboratories, Tel Aviv, Israel), and 1% atropine. Conjunctival peritomy and sclerotomy of 180° (from 0800 to 1400 hours) were performed at the corneoscleral limbus, followed by a two-port vitrectomy. An infusion port was made 1 mm posterior to the sclerocorneal limbus in the inferotemporal quadrant using a small gauge stiletto. A 25-gauge infusion cannula (Accurus Surgical System 25-gauge; Alcon, TX, USA) that delivered balanced salt solution (BSS; Alcon, Japan) was then inserted into the trocar cannula. The second port, created using the same methodology, was used for inserting a vitreous cutter into the superotemporal quadrant. Subsequently, we performed core vitrectomy, and retinal detachment was induced by a subretinal injection of 0.1 ml BSS using a 25G-soft-tip needle (Alcon, TX, USA). All operated eyes were treated with 5% chloramphenciol ointment at the end of the procedure. Clinical examination of the fundus was performed using an indirect ophthalmoscope (Vantage Plus Digital Indirect Ophthalmoscope©, Keeler Ltd., Windsor, UK) 0, 3, 7, 14, and 21 days postoperatively. The operated eyes were examined for the presence of retinal detachment, hemorrhage, and tears as well as lens opacity and other potential complications. Detachment area was quantified at different days postoperatively using ImageJ software (Fiji Imaging processing software http://fiji.sc/Fiji). Statistical analysis was conducted using t-test (SPSS software IBM, NY, USA). […]

Pipeline specifications

Software tools ImageJ, SPSS
Applications Miscellaneous, Microscopic phenotype analysis
Organisms Oryctolagus cuniculus
Diseases Retinal Detachment