Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Jul 2 2013
Last update date: Aug 9 2018
Access: Public
Diseases: Adenocarcinoma, Breast Neoplasms, Neoplasms
Dataset link Microarray-based gene expression data from BPLER tumor explants.

Experimental Protocol


BPLER cells were derived from human primary breast epithelial cells propagated in WIT medium (Ince et al, Cancer Cell, 2007). These cells were transformed with hTERT, SV40 early region and hRASV12 by retroviral transduction. BPLER have a TNBC-like phenotype in vitro and are highly enriched for tumor-initiating cells. To define the phenotype of BPLER-derived tumors in vivo, BPLER cells were injected in the mammary fat-pad of 6 NOD/SCID mice. Once tumors were palpable, mice were sacrificed and tumors excised. Total RNA was extracted using Trizol. The gene expression profile of each tumor was assessed by mRNA microarray analysis. Based on histology and principal component analysis of microarray data, BPLER tumors were remarkably similar to human primary TNBC encountered in the clinic (Petrocca et al, Cancer Cell, 2013).

Repositories


GEO

GSE48444

ArrayExpress

E-GEOD-48444

BioProject

PRJNA210145

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Contact


Tan Ince
Tan A Ince