|Application:||Gene expression microarray analysis|
|Number of samples:||6|
|Release date:||May 11 2015|
|Last update date:||Aug 9 2018|
|Diseases:||Bone Diseases, Metabolic, Nail Diseases, Blood Coagulation Disorders, Inherited, Brachydactyly|
|Dataset link||Distinct gene expression patterns of ‘multipotent' versus ‘unipotent' single colony-derived strains (SCDSs) of human bone marrow stromal cells (BMSCs)|
At least 5x105 cells from each SCDS were homogenized in Trizol (Invitrogen) for RNA extraction. RNA was purified by using a combination of chloroform phase separation and RNeasy Mini Kits (Qiagen) according to the manufacturer's protocol. RNA was quantified and 5ug was processed for microarray analysis (LMT, NCI, Frederick, MD). RNA was reverse transcribed to form cDNA, and hybridized to Affymetrix GeneChip Human Genome U133 Plus 2.0 arrays. Three independent replicates for each of the experimental conditions were carried out and analyzed to control for intra-sample variation.