Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 25
Release date: Sep 21 2018
Last update date: Oct 18 2018
Access: Public
Diseases: Leukemia, Myeloid, Acute
Dataset link Human Bone Marrow Assessment by Single Cell RNA Sequencing, Mass Cytometry and Flow Cytometry [scRNA]

Experimental Protocol


Using standard operating procedures, mononuclear cells from 20 healthy donors' bone marrow aspirates were isolated using Ficoll density gradient separation and cryopreserved in 90% FBS/ 10% DMSO for storage in 72 liquid nitrogen. scRNAseq was performed using 10X Genomics Single Cell 3’ Solution, version 2 according to manufacturer’s instructions (protocol rev A). Libraries were sequenced on HiSeq3000. Droplet-based scRNAseq of bone marrow mononuclear cells for all donor samples was performed with goal minimum sequencing depth of 50,000 reads/cell and detected a mean of 880 genes/cells (range 575-1,390 gene/cell).

Repositories


GEO

GSE120221

ENA

SRP162214

BioProject

PRJNA492227

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Contact


Christopher Hourigan