Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 24
Release date: Aug 12 2009
Last update date: Mar 21 2012
Access: Public
Diseases: Breast Neoplasms, Neoplasms
Dataset link Combinatorial pharmacological approaches target EZH2-mediated gene repression in breast cancer cells

Experimental Protocol


Cells were treated with 2.5 uM DZNep or 5uM AZA for 72 h, and TSA at 100 nM for 24 hours. For AZA treatment, the medium was replaced with freshly added AZA for every 24 h. For co-treatment of cells with DZNep and TSA, DZNep was added for 48 h followed by TSA for an additional 24 h. In total, there were seven different drug treatments (AZA, TSA, DZNep, AZA+TSA, DZNep+AZA, DZNep+TSA, DZNep+AZA+TSA). One group of untreated MCF-7 cells served as the control. Each treatment has 3 technical replicates.

Repositories


GEO

GSE17589

BioProject

PRJNA118523

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Contact


qiang yu