|Application:||RNA-seq analysis, sRNA-seq analysis|
|Number of samples:||4|
|Release date:||Nov 24 2008|
|Last update date:||Mar 20 2012|
|Dataset link||Characterization of unique small RNA populations from rice grain|
In an attempt to gain a broader understanding of gene expression in rice grain, we characterized small RNA populations from Oryza sativa spp. japonica cv. Nipponbare utilizing a deep sequencing approach. Small RNA libraries were constructed from three pools of mature, dormant rice grain and three-week post germination seedlings utilizing RNA-adapter mediated ligation. Each of the four libraries were independently sequenced using high-throughput pyrosequencing. We obtained a total of 679,146 sequences from the three rice grain libraries and 257,394 from one rice seedling library.