|Application:||Gene expression microarray analysis, miRNA array analysis|
|Number of samples:||6|
|Release date:||Aug 31 2015|
|Last update date:||Sep 1 2015|
|Diseases:||Neoplasms, Leukemia, Lymphocytic, Chronic, B-Cell|
|Dataset link||CLL exosomes modulate the transcriptome and behaviour of recipient stromal cells and are selectively enriched in miR-202-3p [mirBase 13]|
miR analysis was carried out on 3 exosomal samples and 3 corresponding cellular samples from CLL patients. Exosomes are a discrete population of small (50-100 nm diameter) EVs of endosomal origin with a lipid membrane bilayer and a cup-shaped morphology. We used common reference design which allows visualization of variations between the samples. Each sample equals one array and each sample compared with common reference (Pool). The pool or common reference was a collection of 23 RNA samples isolated from 23 CLL cases. We hypothesised that CLL derived exosomes should contain unique miRs that are reflective of CLL cell content and additionally relevant for disease biology.