Computational protocol: Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen

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Protocol publication

[…] Nucleotide sequences were analyzed by the nucleotide-nucleotide BLAST (blastn) program []. BLAST pairwise alignments and tree view were generated by using the fast minimum evolution method and maximum sequence difference of 0.75.Protein sequence alignments were performed by the ClustalW software [] and viewed using the Jalview viewer 2.2 []. Conservation index was calculated as described by []. Ole e 1 protein sequences were searched for different motifs with the ScanProsite on-line facilities at the ExPASy Proteomics Server []. […]

Pipeline specifications

Software tools BLASTN, Clustal W, Jalview, ScanProsite
Databases ExPASy
Application Protein sequence analysis
Organisms Olea europaea