|Application:||Gene expression microarray analysis|
|Number of samples:||16|
|Release date:||Nov 21 2015|
|Last update date:||Aug 11 2017|
|Computational protocol:||Agilent Feature Extraction, GeneSpring GX|
|Dataset link||Global gene expression profiling of the effect of probiotics supplementation in dairy cows|
Ten animals were enrolled in the study and an initial blood sample was collected (Day 0). Animals (n=5) received either oral supplement of a commercial probiotic under the name FASTtrak microbial pack (Conklin Company, Kansas City, MO) or water only (control animals) for 60 days. Blood samples were collected at the end of the study from probiotics-treated and control animals for RNA extraction and microarray analysis. In vitro effect of lipopolysaccharide (LPS), endotoxin treatment was evaluated using blood samples collected from probiotics-treated animals (Day 60 samples) to serve as a positive control array. A pooled sample was generated by taking an equal concentration of RNA from experimental animals in each group. Pooled samples from each group were hybridized on Agilent one-color bovine v2 bovine (v2) 4x44K array slides.
DOI: 10.1016/j.gdata.2016.08.016call_split See protocol