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Image analysis of two-dimensional gel electrophoresis is a key step in proteomic workflow for identifying proteins that change under different experimental conditions. Since there are usually large amount of proteins and variations shown in the gel images, the use of software for analysis of 2D gel images is inevitable.
(Li and Seillier-Moiseiwitsch 2011) RegStatGel: proteomic software for identifying differentially expressed proteins based on 2D gel images. Bioinformation.