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SDA / Statistical DIGE Analyzer

A package for the statistical analysis of data from two-dimensional difference gel electrophoresis (DIGE). The SDA comprises the following features: (i) simultaneous differential analyses of thousands of protein spots: t-tests, fold change (plus respective confidence intervals), p-value adjustements, (ii) power analysis for sample size calculations, (iii) principal component analysis for comparing between gel variation, (iv) analysis of 'minimal' and 'saturation' designed studies and (v) calibration of raw spot intensities.

HUVEC / Human Umbilical Vein Endothelial Cells

Purposes the proteomic analysis of the most explored present endothelial cell model, i.e. primocultures of human umbilical vein endothelial cells (HUVEC). The data on HUVEC proteomics are depicted in the 2D gels and identifications area (identification, number of accession in database, pI and MW…). This database offers the proteomic study of HUVEC using the classical approach combining two-dimensional electrophoresis (2-DE) and mass spectrometry (MALDI-TOF and LC-ESI-MS/MS) after peptide mass fingerprinting (PMF).