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MIST / Microscopy Image Stitching Tool
Serves for stitching of large 2D time-lapse mosaics. MIST can estimate the mechanical stage model parameters from the computed translations to limit the optimization search space. It also minimizes stitching errors by constraining and optimizing the translations within a square area. It can be used on any grid-based dataset like those acquired by a microscope. Moreover, this tool utilizes normalized cross correlations to compute image registrations.
Applies a recursive normalization algorithm to stitch large-scale immunofluorescence-based mosaics without incurring vignetting seams. QuickStitch works successfully for tissues of differing size, morphology, and fluorescence intensity. It requires no specific information about the imaging system. It is provided as an open-source tool that is both user friendly and extensible, allowing straightforward incorporation into existing image processing pipelines. This enables studies that require accurate segmentation and analysis of high resolution datasets when parameters of interest include both cellular-level phenomena and larger tissue-level regions of interest.
Amira 3D Software for Life Sciences
Allows users to visualize, manipulate, and understand data from imaging modalities such as computed tomography, microscopy or Magnetic resonance imaging (MRI). Amira 3D Software for Life Sciences provides features to import and process 2D and 3D images data, visualization techniques and tools for visual analysis. Users can also create and share presentations. The base product can be customized by adding functional extensions to fit special needs in different application areas.
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