Computational protocol: Mitochondrial Biogenesis Drives a Vicious Cycle of Metabolic Insufficiency and Mitochondrial DNA Deletion Mutation Accumulation in Aged Rat Skeletal Muscle Fibers

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Protocol publication

[…] The Affymetrix GCOS software package was used to identify up-regulated genes. Genes that are called present from the ETS abnormal or control chips are considered specific to the population of cells from which they came and differentially expressed unless they are detected in both ETS and control samples. Additionally, genes whose expression is greater than a ‘two fold-change’ are also considered to be differentially-regulated. Genes were annotated based upon NetAffx Annotation Release 21. Gene Ontology analysis was performed using the DAVID Bioinformatic database , . […]

Pipeline specifications

Software tools NetAffx, DAVID
Application Protein sequence analysis
Organisms Rattus norvegicus