Dataset features

Specifications


Dataset type: Expression profiling by high throughput sequencing
Number of samples: 34
Release date: Feb 13 2019
Last update date: Feb 15 2019
Access: Public
Dataset link Virus-specific memory T cells populate tumors and can be repurposed for tumor immunotherapy

Experimental Protocol


For B16 mouse tumor studies, B16 tumors in OT-1 chimeras were treated with relevant or irrelevant viral peptide. 9 hours after peptide delivery, B16 tumors were harvested from 3 mice per group. RNA was isolated from tumor homogenate for sequencing. For Braf/Pten tumor studies, murine melanoma tumors in OT-1 chimeras were isolated, sliced into thin sections, and incubated with relevant or irrelevant peptide ex vivo. RNA was isolated from tumor culture homogenate for sequencing. For human tumor samples, tumors were sliced into thin sections, and incubated with relevant or irrelevant viral peptides ex vivo. RNA was isolated from tumor culture homogenate for sequencing.

Repositories


GEO

GSE124620

ENA

SRP175164

BioProject

PRJNA512856

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Contact


Sathi Wijeyesinghe