Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 17
Release date: May 18 2017
Last update date: May 4 2018
Access: Public
Chemicals: Zinc
Dataset link Gene expression of fly testes with dMi-2, kumgang (CG5204) knock downs

Experimental Protocol


RNA was obtained from whole testes of flies with following mutations: 1) UAS-RNAi-kmg (VDRC# 107395); TM6b (kmg_Ctrl1/2.CEL), 2) UAS-RNAi-kmg (VDRC# 107395); bam-gal4, UAS-dicer2 (kmg_KD1/2.CEL), 3) UAS-RNAi-kmg(VDRC# 107395); bam-gal4, aly[2]/aly[5p] (kmg_KD_aly1/2.CEL), 4) UAS-RNAi-dMi-2 (VDRC# 107204); TM6b (dMi2_Ctrl1/2.CEL), 5) UAS-RNAi-dMi-2 (VDRC# 107204); bam-gal4, UAS-dicer2 (dMi2_KD1/2.CEL). Each genotype had two biological replicates. All experiments were done in 30C. RNA was obtained from whole testes of flies. Genotypes of flies and treatment applied to those lines are like the following: 1) w; ;bam[1]/bam[delta86] (no heat shock, 1 sample), 2) w; ;bam[1]/bam[delta86] (24 hours after 30 minutes pulse heat shock, 1 sample), 3) hs-Bam;;bam[1]/bam[delta86] (no heat shock, 2 samples), 4) hs-Bam;;bam[1]/bam[delta86] (24 hours after 30 minutes pulse heat shock, 2 samples). Flies were raised in 25C.

Repositories


GEO

GSE68696

BioProject

PRJNA283406

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Contact


Margaret Fuller