Dataset features

Specifications


Dataset type: Other
Number of samples: 71
Release date: Dec 12 2017
Last update date: Feb 26 2018
Access: Public
Dataset link Massively parallel reporter assay of 3’UTR sequences identifies in vivo rules for mRNA degradation

Experimental Protocol


temporal expression profiles of reporter mRNAs from zebrafish embryos (7 replicates total) or oocytes (2 replicates total). A total of five embryo replicates were collected using pre-adenylated (A+) reporters in three separate experiments. In the first experiment, a sample was collected every hour between 1h to 8h, and at 10h. In the second experiment, a single sample was collected every hour between 1h to 10h, and split into two after RNA extraction to produce two technical replicates (techrep). In the third experiment, two separate samples were collected every two hours between 2h to 10h to produce two same-day biological replicates (biorep). A total of two replicates were collected using non-adenylated (A-) reporters in two separate experiments. In the first experiment, a sample was collected every hour between 1h to 8h, and at 10h. In the second experiment (rep), samples were collected at 1h, 2h, 3h, 4h (3 samples), 6h, 8h and 10h.

Repositories


GEO

GSE106677

ENA

SRP124609

BioProject

PRJNA417597

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Contact


Michal Rabani