Computational protocol: Crystal structure and biochemical analyses reveal Beclin 1 as a novel membranebinding protein

Similar protocols

Protocol publication

[…] All data were collected at the SPring-8 beamline BL41XU, integrated and scaled using the HKL2000 package . Further processing was carried out using programs from the CCP4 suite . Data collection statistics are summarized in . The selenium positions were determined using the program SHELXD . The identified sites were refined and initial phases were generated in the program PHASER with the SAD experimental phasing module. Solvent flattening and histogram matching were performed using DM . The initial model was traced automatically using the program BUCCANEER and was manually rebuilt in COOT . The final structure was refined with PHENIX . […]

Pipeline specifications

Software tools CCP4, SHELX, Buccaneer, Coot, PHENIX
Application Protein structure analysis
Diseases Neoplasms
Chemicals Cardiolipins