Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 18
Release date: Jan 7 2011
Last update date: Mar 22 2012
Access: Public
Diseases: Brain Neoplasms, Neoplasms, Neuroblastoma
Dataset link Glycosyl-T expression in NB cells

Experimental Protocol


The effect of retinoic acid on the mRNA levels of ganglioside glycosyltransferases in neuroblastoma cells in vitro Experiment to determine mRNA expression for ganglioside glycosyltransferases in NB cells (untreated versus treated with ATRA) using the Glyco-gene Chip by the Consortium for Functional Glycomics. Human NB cell lines LAN-5 were incubated with 10 ┬ÁM of all-trans retinoic acid or untreated as control, and total RNA harvested after 24 hours, 72 hours and 120 hours of exposure to retinoic acid. We performed three independent experiments on either cell line so that for each cell line and each time point three replicates were be obtained (total: 18 chips). Glycosyltransferases of particular interest are LacCer synthase, GlucCer synthase, GM3 synthase, GD3 synthase, GM2/GD2 synthase, GD1b/GM1a synthase, GT1b/GD1a synthase and GQ1b/GT1a synthase.

Repositories


GEO

GSE26481

ArrayExpress

E-GEOD-26481

BioProject

PRJNA136505

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Contact


Steven Head