Computational protocol: Neurodevelopmental alterations and seizures developed by mouse model of infantile hypophosphatasia are associated with purinergic signalling deregulation

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Protocol publication

[…] At E14.5, pregnant C57BL/6 female mice were anaesthetized by continuous inhalation of Isoflurane (Baxter, Deerfield, US). The abdomen was opened and the uterine horns exposed. The retrovirus stock preparation containing 0.03% fast green was injected into one lateral ventricle of each embryo using a pulled glass micropipette. The wall and skin of the abdominal cavity were sutured and the embryos were allowed to develop normally until P9. Retroviral stock preparation was prepared as indicate in Supplementary Material.The spine number and dendritic branching (number of primary, secondary and tertiary branches) of individual neurons of the somatosensory cortex or DG were measured with ImageJ and NeuronJ software in confocal reconstructions. Measu┬árements were only made on cortical neurons with the main apical process parallel to the plane of section contacting layer I, and with at least three basal processes. […]

Pipeline specifications

Software tools ImageJ, NeuronJ
Application Microscopic phenotype analysis
Organisms Mus musculus, Homo sapiens
Diseases Bone Diseases, Metabolic, Hypophosphatasia
Chemicals Adenosine Triphosphate, Vitamin B 6