Dataset features

Specifications


Application: BS-seq analysis
Number of samples: 15
Release date: Sep 5 2018
Last update date: Oct 12 2018
Access: Public
Taxon: Bos taurus
Dataset link In utero heat stress alters the offspring epigenome [methylation]

Experimental Protocol


Nine bull calves and six heifers gestated under maternal conditions of heat stress (HT, dams had access to shade) or cooling (CL, dams had access to shade, fans and soakers) were used for this study. Both bull calves (HT = 4 and CL = 5) and heifers (HT = 3 and CL = 3) were exposed to the maternal treatment through the intrauterine environment during the last 6-7 weeks of fetal development. Liver samples were collect from bull calves inmediately after birth. Heifers were managed under identical conditions from birth through their first lactation and mammary gland samples were collected on day 21 of the first lactation, coinciding with the rising milk yield phase of the lactation cycle. Liver and mammary gland samples were subjected to DNA extraction and subsequent reduced representation bisulfite sequencing.

Repositories


GEO

GSE119445

ENA

SRP159548

BioProject

PRJNA489263

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Contact


Francisco Peñagaricano