Computational protocol: Expression of Phi11 Gp07 Causes Filamentation in Escherichia coli

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Protocol publication

[…] Genome databases in NCBI ( and Pfam ( were used for bioinformatics analysis of Gp07. EMBOSS programs ( were used for different bioinformatics analyses like molecular weight determination, charge and presence of different amino acid. Sequence similarity search was carried out by BLAST server ( The search for Phi11 gp07 gene was carried out using BLASTP in different databases in Caudovirales order and 50% or more identity was taken as the threshold []. Sequences similar to the gp07 of Phi11 were detected from different non-redundant (NR) protein sequence databases by BLAST (blastp) program 2.2.32+. Alignment of full-length Gp07 protein was performed by Clustal Omega program 1.2.1 ( Using all the proteins which shared 50% identity to the Phi11 Gp07, a dendrogram was constructed using ClustalW2 Phylogeny program ( with neighbor-joining method. […]

Pipeline specifications

Software tools EMBOSS, BLASTP, Clustal Omega, Clustal W
Databases Pfam
Application Phylogenetics
Organisms Escherichia coli
Chemicals Amino Acids