Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 39
Release date: Sep 7 2015
Last update date: Feb 21 2018
Access: Public
Diseases: Leukemia, Leukemia, Hairy Cell, Leukemia, Myeloid, Acute
Dataset link Both gain and loss of function of miR-126 promote t(8;21) leukemia progression with different consequences and through different mechanisms

Experimental Protocol


A total of 39 mouse bone marrow samples including 36 mouse AML samples with AE9a, AE9a+miR-126, and miR-126KO+AE9a collected from the 1st, 2nd, 3rd and 4th passages of BMT recipient mice (3 mice for each group in each passage), along with 3 normal controls from the 1st passage of BMT, were analyzed by use of Affymetrix GeneChip Mouse Gene 2.0 ST Array (Affymetirx, Santa Clara, CA). For each sample, the CD45.1+ cells (i.e., transplanted donor cells) were sorted with flow cytometry from whole BM cells collected from BMT recipient mice at the end stage. Then total RNA was isolated by use of miRNeasy extraction kit (Qiagen, Valencia, CA).

Repositories


GEO

GSE65939

ArrayExpress

E-GEOD-65939

BioProject

PRJNA275444

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Contact


Jianjun Chen