Dataset features

Specifications


Application: BS-seq analysis
Number of samples: 17
Release date: Dec 18 2013
Last update date: Jan 24 2018
Access: Public
Diseases: Carcinoma, Non-Small-Cell Lung, Lung Neoplasms
Dataset link Examination of genome-wide methylation changes in lung cancer cell lines A549 (A) and HTB56 (H) [RRBS-Seq experiments]

Experimental Protocol


We studied genome-wide methylation changes in lung cancer cell lines A549 (A) and HTB56 (H). We generated NSCLC lines with highly increased propensity to form tumor nodules in murine lungs after intravenous injections. In addition to the normal cell lines (0R) we analyzed the methylome of the the cell lines after three rounds of in vivo selection towards a highly metastatic phenotype (3R). Next we studied changes in the methylome of highly metastatic cell lines after DNA Methyltransferase inhibition by 5-Azacytidine treatment at low concentrations (250 nM & 1000 nM) for 6 days. During treatment cells were supplemented with fresh medium every 48 hours. After 6 days of 5-Azacytidine exposure, cells were washed three times with PBS to wash out the drug. The cells were released for additional 7 days in regular medium. We followed up the DNA methylation changes at day 13 of the experiment.

Repositories


GEO

GSE52140

ArrayExpress

E-GEOD-52140

ENA

SRP033242

BioProject

PRJNA227472

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Contact


Christian Rohde