Dataset features

Specifications


Application: Gene expression microarray analysis, DNA methylation array analysis
Number of samples: 10
Release date: Nov 12 2009
Last update date: Oct 29 2018
Access: Public
Genes: DNMT1
Taxon: Homo sapiens
Dataset link DNMT1 Maintains Progenitor Function in Self-Renewing Somatic Tissue

Experimental Protocol


Gene expression analysis: To establish a differentiation signature for primary human keratinocytes, total RNA was isolated in biologic duplicate from cells cultured in growth conditions and high calcium differentiation conditions and hybridized to Affymetrix HG-U133 2.0 Plus arrays. This gene signature was also compared to DNMT1 deficient cells cultured in growth conditions. Methylated DNA profiling: To globally characterize DNA methylation in primary human keratinocytes, genomic DNA was immunoprecipitated using a 5-methylcytidine antibody, amplified, and hybridized to NimbleGen HG18 promoter tiling arrays. Profiling was done using DNA isolated in growth conditions as well as differentiation conditions.

Repositories


GEO

GSE18590

ArrayExpress

E-GEOD-18590

BioProject

PRJNA120305

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