|Application:||Gene expression microarray analysis, DNA methylation array analysis|
|Number of samples:||10|
|Release date:||Nov 12 2009|
|Last update date:||Oct 29 2018|
|Dataset link||DNMT1 Maintains Progenitor Function in Self-Renewing Somatic Tissue|
Gene expression analysis: To establish a differentiation signature for primary human keratinocytes, total RNA was isolated in biologic duplicate from cells cultured in growth conditions and high calcium differentiation conditions and hybridized to Affymetrix HG-U133 2.0 Plus arrays. This gene signature was also compared to DNMT1 deficient cells cultured in growth conditions. Methylated DNA profiling: To globally characterize DNA methylation in primary human keratinocytes, genomic DNA was immunoprecipitated using a 5-methylcytidine antibody, amplified, and hybridized to NimbleGen HG18 promoter tiling arrays. Profiling was done using DNA isolated in growth conditions as well as differentiation conditions.