|Application:||Gene expression microarray analysis|
|Number of samples:||6|
|Release date:||Dec 1 2008|
|Last update date:||Aug 28 2018|
|Diseases:||Breast Neoplasms, Neoplasms|
|Dataset link||Breast cancer-associated fibroblasts confer AKT1-mediated epigenetic silencing of Cystatin M in epithelial cells.|
MCF10A cells were cocultured with different breast fibroblasts that extracted from either breasttumors or normal breast tissues. After three weeks coculture, MCF10A cells were sorted and harvested for total RNA extraction. Affymetrix expression microarray was performed to analyze gene expression changes. Neither replicates nor dye-swaps was carried out.