Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 14
Release date: Feb 8 2012
Last update date: Feb 21 2017
Access: Public
Chemicals: Fatty Acids, Fluconazole, Oleic Acid
Dataset link Gene expression response to the antifungal compound 6-Nonadecynoic acid (6-NDA) in Saccharomyces cerevisiae and Candida albicans

Experimental Protocol


Cultures of S. cerevisiae strain S288C were started at OD600 of 0.1, allowed to grow to OD600 of 0.2, then treated with either DMSO (0.25%) or 6-NDA at the IC50 concentration (2.2 ug/ml). When OD600 of 0.5 was reached, cells were harvested and frozen. Two biological replicate cultures were grown for each treatment, and RNA from each replicate was hybridized to 2 independent arrays, resulting in 4 hybridizations for each treatment. Cultures of C. albicans strain SC5314 were started at OD600 of 0.1, allowed to grow to OD600 of 0.2, then treated with either DMSO (0.25%) or 6-NDA at the IC50 concentration (4 ug/ml). When OD600 of 0.5 was reached, cells were harvested and frozen. Three biological replicate cultures were grown for each treatment, and RNA from each replicate was hybridized as dye-swap pairs, resulting in 6 hybridizations for each treatment.

Repositories


GEO

GSE35604

ArrayExpress

E-GEOD-35604

BioProject

PRJNA152529

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Contact


Ameeta Agarwal