|Application:||Gene expression microarray analysis|
|Number of samples:||60|
|Release date:||Feb 2 2009|
|Last update date:||Dec 6 2012|
|Chemicals:||Carbohydrates, Nucleotides, Oxygen, Steroids|
|Dataset link||Hypoxia alters gene expression in the gonads of zebrafish|
Reproductively mature zebrafish (Danio rerio; ab wild-type strain; 7 months old) were obtained from an on-site culture unit. Mixed groups of four males and four females were randomly loaded into 10 L tanks, receiving a continuous flow of Lake Superior water. Zebrafish were exposed to hypoxia for duration of 4 and 14 days. There were three oxygen treatments: normoxia (6.5 mg dissolved O2/L), moderate hypoxia (3 mg dissolved O2/L) and severe hypoxia (1 mg dissolved O2/L). Each exposure tank contained four males and four females, and each time and oxygen content treatment combination was replicated twice (i.e., fish were held in two separate exposure tanks). The 4 and 14 d exposures were conducted concurrently. Aliquots of gonadal RNA from male and female fish exposed to hypoxia for 4 and 14 days (five biological replicates from each of the three oxygen treatment groups) were submitted to an Agilent certified facility, MOgene, LC (St. Louis, MO), for gene expression profiling.