|Application:||Gene expression microarray analysis|
|Number of samples:||54|
|Release date:||Jun 30 2012|
|Last update date:||May 10 2018|
|Taxon:||Homo sapiens, Mus musculus|
|Dataset link||Parallel selection mapping using artificially selected mice reveals body weight control loci (gene expression)|
Mouse tissue samples (muscle and liver) were collected from 44 and 45 days old males from each of the nine out of thirteen lines (5 High lines: BEHi, DUHi, EDHi, MUHi, ROHi and 4 Control lines: BELi, EDLi, MULi and ROLi; n=23 total) at the University of Edinburgh. Frozen tissue samples were stored at -80ºC until use. Tissues were dissociated using TissueLyser II with steel beads (both Qiagen) following manufacturers’ instructions. mRNA was extracted and purified using TRIzol Reagent and the PureLink RNA Mini Kit (Life Technologies GmbH, Darmstadt, Germany). cDNA was synthesized using Maxima First Strand cDNA synthesis kit (Thermo Fisher Scientific, St. Leon-Rot, Germany). Muscle and liver cDNA samples were hybridized on a Mouse GE 4x44k Microarray (Agilent Technologies, Inc., Waldbronn, Germany) according to manufacturer’s recommendations. Three technical replicate each was done on pooled cDNA samples from each mouse line.
Yingguang Frank Chan