Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 54
Release date: Jun 30 2012
Last update date: May 10 2018
Access: Public
Genes: ADGRD1, PRLHR
Taxon: Homo sapiens, Mus musculus
Dataset link Parallel selection mapping using artificially selected mice reveals body weight control loci (gene expression)

Experimental Protocol


Mouse tissue samples (muscle and liver) were collected from 44 and 45 days old males from each of the nine out of thirteen lines (5 High lines: BEHi, DUHi, EDHi, MUHi, ROHi and 4 Control lines: BELi, EDLi, MULi and ROLi; n=23 total) at the University of Edinburgh. Frozen tissue samples were stored at -80ºC until use. Tissues were dissociated using TissueLyser II with steel beads (both Qiagen) following manufacturers’ instructions. mRNA was extracted and purified using TRIzol Reagent and the PureLink RNA Mini Kit (Life Technologies GmbH, Darmstadt, Germany). cDNA was synthesized using Maxima First Strand cDNA synthesis kit (Thermo Fisher Scientific, St. Leon-Rot, Germany). Muscle and liver cDNA samples were hybridized on a Mouse GE 4x44k Microarray (Agilent Technologies, Inc., Waldbronn, Germany) according to manufacturer’s recommendations. Three technical replicate each was done on pooled cDNA samples from each mouse line.

Repositories


GEO

GSE36443

ArrayExpress

E-GEOD-36443

BioProject

PRJNA155829

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Contact


Yingguang Frank Chan