Dataset features


Dataset type: Expression profiling by RT-PCR, Transcriptome or Gene expression
Number of samples: 12
Release date: Dec 27 2017
Last update date: Mar 28 2018
Access: Public
Dataset link Engineered glycocalix regulates stem cell proliferation in murine crypt organoids

Experimental Protocol

Lgr5+ mouse stem cells were sorted from dissociated organoids that had been treated with in situ fucosylation for 4 days or untreated control RNA was isolated and analyzed, quality was determined using a spectrophotometer and was reverse transcribed using a cDNA conversion kit. The cDNA was used on the real-time RT2 Profiler PCR Array (QIAGEN, Cat. no. PAMM-405Z) in combination with RT2 SYBR® Green qPCR Mastermix (Cat. no. 330529). The average fold-change of gene expression in stem cells was analyzed for untreated vs. glycan modified organoids from six biological replicates for 84 genes. CT values were exported to an Excel file to create a table of CT values. This table was then uploaded on to the data analysis web portal at Samples were assigned to controls and test groups. CT values were normalized based on a/an Manual Selection of reference genes.