Computational protocol: Dispersal Routes and Habitat Utilization of Juvenile Atlantic Bluefin Tuna, Thunnus thynnus, Tracked with Mini PSAT and Archival Tags

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Protocol publication

[…] We deployed mini PSATs (X-tags) in August – October 2007 (n = 32), 2008 (n = 22) and 2009 (n = 4) on ABFT (105–168 cm CFL, ) off Cape Cod (Massachusetts, USA, ). All tagging was conducted aboard the fishing vessels by rod and reel. Fish were brought on board and a wet towel placed over the eyes and measured for curved fork length (CFL) and finlet samples were taken for further genetic analysis and determination of natal origin. Tag and tether assemblies, materials and tagging procedures were similar to those previously described for adult ABFT , , with tether length adjusted for smaller fish. Tags were attached by inserting the dart near the base of the second dorsal fin. In 2007, we experimented with a secondary attachment to reduce tag movement during swimming (n = 16). This consisted of a short length of monofilament in a crimped loop, attached to a small white nylon dart. T-tests indicated this did not make a significant difference in attachment duration (days attached with tether, x = 197 days, no tether, x = 201 days, p = 0.9477), and the practice was discontinued in subsequent years. We performed all field work under National Oceanic and Atmospheric Administration exempted fishing permits TUNA-EFP-07-01, TUNA-EFP-08-03, TUNA-EFP-09-03 for years 2007, 2008 and 2009, respectively.The mini PSATs used in this study were programmed to release after 12 months and to record external temperature and pressure (depth) every 15 minutes. All tags had a failsafe release set at 4 days, which would indicate post-release mortality or premature tag release. As the tags continued to be developed and improved during this study, the manufacturer changed several programming settings. In 2007, X-tags capabilities mirrored that of the manufacturer's standard size PTT-100 tag. This is described in detail elsewhere and on the manufacturer's website ( X-tags deployed after 2007 recorded light, external temperature (0.01 C°) and depth (0.33 m) every two minutes in a separate part of the memory, accessible if the tag is recovered. Additionally, X-tags manufactured after 2007 have a variable depth measurement precision as follows: readings above 86 m  = 0.67 m, 258–86 m  = 1.34 m, 602–258 m  = 2.69 m and 602–129 1m  = 5.38 . The differences resulted from an increase from 8-bit to 12 bit memory, allowing more divisions between the minimum and maximum depth (i.e. 256 divisions for 8-bit memory and ∼1377 m maximum depth yields 5.38 m accuracy). This was a major improvement over the standard 5.38 m resolution possible in tags deployed through 2007. While the PTT-100 has diode placement allowing 360 degrees of light sensing in the nosecone, the X-tags light sensor was located in the body of the tag. […]

Pipeline specifications

Software tools DART, TUNA
Application GWAS