Computational protocol: Engineering Yeast Hexokinase 2 for Improved Tolerance Toward Xylose-Induced Inactivation

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Protocol publication

[…] A homology model of S. cerevisiae Hxk2p was generated based on the closed conformation of the S. cerevisiae Hxk1p scaffold with a glucose moiety in the active site (PDB: 3B8A_X). The models were generated using SWISS-MODEL by submitting the Hxk2p (GenBank: EIW10681.1) and the later obtained Hxk2p-Y amino acid sequences for automatic homology modelling. Structural analysis of the model was done manually using PyMOL (http://www.pymol.org). Further information regarding the structural analysis is described in the Results section. […]

Pipeline specifications

Software tools SWISS-MODEL, PyMOL
Application Protein structure analysis
Organisms Saccharomyces cerevisiae
Chemicals Glucose, Tyrosine, Xylose