Computational protocol: Cloning and expression analysis of two distinct HIF-alpha isoforms – gcHIF-1alpha and gcHIF-4alpha – from the hypoxia-tolerant grass carp, Ctenopharyngodon idellus

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Protocol publication

[…] Phylogenetic analysis was performed by maximum parsimony using the PROTPARS program of the PHYLIP package version 3.57 c []. Support for the inferred clades was obtained by bootstrap analysis from 1000 replications of the data set using the SEQBOOT and CONSENSE programs. Phylogenetic tree was displayed using TREEVIEW []. Sequence analyses and homology searches were performed using the online BLAST suite of programs (NCBI, USA). […]

Pipeline specifications

Software tools PHYLIP, TreeViewX
Application Phylogenetics
Organisms Ctenopharyngodon idella
Diseases Muscular Diseases, Fractures, Stress
Chemicals Amino Acids