|Application:||Gene expression microarray analysis|
|Number of samples:||16|
|Release date:||Dec 21 2015|
|Last update date:||Apr 5 2017|
|Diseases:||Leukemia, Multiple Myeloma, Myelodysplastic Syndromes, Leukemia, Myeloid, Acute|
|Dataset link||Functional abnormalities and changes in gene expression in fibroblasts and macrophages from the bone marrow of patients with acute myeloid leukemia|
Analysis of gene expression in fibroblast and macrophage populations isolated from primary long-term bone marrow cultures (LTBMC). Adherent layers of LTBMCs were trypsinized, fibroblasts and macrophages were purified using anti-Fibroblast or CD14 MicroBeads (Miltenyi Biotec, Begisch-Gladbach, Germany), and total RNA was extracted by using the RNeasy kit from Qiagen (Hilden, Germany), as specified by the manufacturer. Global gene expression was analyzed in 4 samples each of fibroblasts and macrophages from AML patients and control subjects.