Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 16
Release date: Dec 21 2015
Last update date: Apr 5 2017
Access: Public
Diseases: Leukemia, Multiple Myeloma, Myelodysplastic Syndromes, Leukemia, Myeloid, Acute
Dataset link Functional abnormalities and changes in gene expression in fibroblasts and macrophages from the bone marrow of patients with acute myeloid leukemia

Experimental Protocol


Analysis of gene expression in fibroblast and macrophage populations isolated from primary long-term bone marrow cultures (LTBMC). Adherent layers of LTBMCs were trypsinized, fibroblasts and macrophages were purified using anti-Fibroblast or CD14 MicroBeads (Miltenyi Biotec, Begisch-Gladbach, Germany), and total RNA was extracted by using the RNeasy kit from Qiagen (Hilden, Germany), as specified by the manufacturer. Global gene expression was analyzed in 4 samples each of fibroblasts and macrophages from AML patients and control subjects.

Repositories


GEO

GSE26294

BioProject

PRJNA135055

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Contact


Ludger Klein-Hitpass