Computational protocol: Circadian Desynchrony Promotes Metabolic Disruption in a Mouse Model of Shiftwork

Similar protocols

Protocol publication

[…] Microarray experiments using liver and adipose tissue total RNA preparations were performed at the Transcriptome Analysis Lab (TAL) of the University of Göttingen, Germany. cRNA samples were hybridized to MoGene ST1.0 gene expression arrays (Affimetrix, Santa Clara, CA). Three chips per time point, condition and tissue were used. Gene expression was normalized using d-Chip Software and rhythmic gene expression and expression phases were determined using CircWave Software (University of Groningen, The Netherlands) as described . Rhythmic genes were grouped and heat maps were generated using d-Chip Software . These sequence data have been submitted to the GEO databases under accession number GSE33381. Gene ontology analysis was performed using DAVID (NIAID, NIH) , . […]

Pipeline specifications

Software tools CircWaveBatch, DAVID
Applications Gene expression microarray analysis, Transcription analysis
Organisms Mus musculus, Homo sapiens
Diseases Metabolic Diseases, Chronobiology Disorders
Chemicals Glucose