|Application:||Gene expression microarray analysis|
|Number of samples:||21|
|Release date:||Dec 31 2014|
|Last update date:||May 11 2017|
|Dataset link||Granulocyte-monocyte progenitor cell cycle regulation occurs through calcium flux and calcineurin-NFAT signaling via Flt3-L|
Bone marrow from C57BL/6 mouse depleted for lineage positive differentiated cells (lin-, CD3e-, B220-, CD19- CD11b-, CD11c-, Gr-1-, TER-119-, NK1.1- with addition of CD127-) and enriched for c-kit. lineage negative, cKIT positive progenitors were cultured in IMDM (containing 200ng/ml mrFLT3-L (Stem cells), 10% heat-inactivated FCS (Life Technologies), streptomycin 100mg/ml and penicillin 100 U/ml) at 3x106 cells/ml. For culture of sorted progenitors, stem cell factor (SCF; 50ng/ml), IL-6 (20ng/ml) and IL-3 (10ng/ml; all R&D Systems) were added (referred to as HSC medium). 2µg/ml CsA or 0.2µg/ml FK506 (Cell signalling Technology) were added to the cell culture for 30 mins preceding the addition of Flt3-L were maintained throughout the culture.
Citations per year
Stem Cells (Dayton, Ohio)