Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 30
Release date: Jan 20 2010
Last update date: Oct 29 2018
Access: Public
Diseases: Breast Neoplasms
Dataset link Human Negative Elongation Factor Activates Transcription and Regulates Alternative Transcription Initiation

Experimental Protocol


Gene expression analysis of RNA samples from NELF-depleted T47D breast cancer cell line. We analyzed gene expression of T47D cells transfected with siRNAs for control and each of the four NELF subunits by exon array. Each knockdown condition contains three technical repeats. Differentially expressed genes and exons between knockdown of the individual NELF subunits and the control are analyzed by the Arrayassist software from Stratagene. The commonly affected genes and exons among all four NELF subunits knockdown samples are chosen for further studies.

Repositories


GEO

GSE19940

BioProject

PRJNA122633

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Contact


Jianlong Sun