|Application:||Gene expression microarray analysis|
|Number of samples:||24|
|Release date:||Jul 20 2013|
|Last update date:||Jul 20 2013|
|Dataset link||Mechanistic information on perfluorooctanoic acid (PFOA) exposure in zebrafish (Danio rerio)|
Adult zebrafish (Danio rerio) were exposed to nominal concentrations of 0mg/l; 0.1mg/l; 1mg/l PFOA (perfluorooctanoic acid) for 28 days. Three different 25 litre aquaria per exposure concentration were used resulting in 3 biological replicates with each aquarium containing 8 male and 8 female zebrafish. The livers of 6 male fish and 6 female fish were pooled separately and snap frozen in liquid nitrogen. A reference sample was made by pooling equal amounts of RNA from all samples. A carriage wheel design was used in which all samples were connected to the reference sample and the main contrasts of interest were made directly on the same microarrays as frequently as possible. This design resulted in technical triplicates of each sample.