Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 54
Release date: May 20 2016
Last update date: Aug 13 2018
Access: Public
Diseases: Breast Neoplasms
Chemicals: Cholesterol, Estrogens, Tamoxifen
Dataset link Cholesterol biosynthesis pathway as a novel mechanism of resistance to estrogen deprivation in estrogen receptor positive breast cancer

Experimental Protocol


To identify common adaptive mechanisms associated with resistance to aromatase inhibitors (AI), we assessed changes in global gene expression during adaptation to long-term estrogen deprivation (LTED) in a panel of ER+ BC cell lines cultured in 2D on plastic (MCF7, T47D, HCC1428, SUM44 and ZR75.1) or 3D on collagen (MCF7) to model the stromal compartment. Furthermore, dimethyl labelling followed by LC-MS/MS was used to assess global changes in protein abundance. The role of target genes/proteins on proliferation, ER-mediated transcription and recruitment of ER to target gene promoters was analysed

Repositories


GEO

GSE75971

BioProject

PRJNA305878

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Contact


Qiong Gao
Alice Gao

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